Herbicide tolerant, insect resistant corn

A stacked maize line DAS-Ø15Ø7-1 x MON-ØØ81Ø-6 x SYN-IR162-4 x MON-ØØ6Ø3-6 obtained through conventional breeding of each of the parental organisms. The modifications in these lines confer tolerance to the glyphosate and glufosinate herbicides and resistance to Lepidoptera and European Corn Borer pests.

pNOV1300, PV-ZMGT32, PV-ZMBK07, PV-ZMGT10 and PHI8999A

DNA insert from MON810 vector PV-ZMBK07 and PV-ZMGT10:
The cry1Ab gene, isolated from the common soil bacterium Bacillus thuringiensis (Bt), produces the insect control protein Cry1Ab, a delta-endotoxin.

DNA insert from NK603 vector PV-ZMGT32:
59122 x NK603 also contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate. The glyphosate-tolerant EPSPS gene was isolated from the CP4 strain of the common soil bacterium Agrobacterium tumefaciens.

DNA insert from TC1507 vector PHI8999A:
Vector contains the cry1F gene from Bacillus thuringiensis to confer resistance to the European corn borer. The vector additionally contains the pat gene to confer tolerance to the glufosinate ammonium herbicide.

DNA insert from MIR162 vector pNOV1300:
MIR162 maize is transformed with vip3Aa20 gene which encodes the Vip3Aa20 protein that confers resistance against lepidopteran insect pests. Event MIR162 maize also contains the manA gene from Escherichia coli, which encodes the selectable marker, phosphomannose isomerase (PMI).

For additional information on this LMO, please refer to the records of the parental LMOs.