Record details

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Living Modified Organism identity

The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name

Canola modified for herbicide tolerance

Transformation event

aroA

Developer(s)

Record #106248

Анатолий Николаевич Евтушенков

Head of the Department of Molecular Biology

Department of Molecular Biology

Faculty of Biology of the Belarusian State University

Kurchatova, 10

Minsk

Belarus

Phone:	+375 17 2095898
Email:	evtushenkov@bsu.by

Description

Transgenic rapeseed line with an integrated, modified, aroA gene to confer resistance to the herbicide glyphosate.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #12083

Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA

Characteristics of the transformation process

Vector

pBI121

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Introduced or modified genetic elements

Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.

Record #100270

Nopaline Synthase Gene Promoter - Agrobacterium tumefaciens - Agrobacterium

Record #15001

Neomycin Phosphotransferase II - Escherichia coli - ECOLX

Resistance to antibiotics - Kanamycin

Record #100287

CaMV 35S promoter - Cauliflower mosaic virus - CaMV

Record #100269

Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium

Record #106246

5-enolpyruvylshikimate-3-phosphate synthase gene - Dickeya dadantii - DICDA

Resistance to herbicides - Glyphosate

Record #106247

Chloroplast Transit Peptide - Nicotiana tabacum - Tobacco, TOBAC

Notes regarding the genetic elements introduced or modified in this LMO

Transgenic rapeseed line with integrated gene aroA under control of the constitutive 35S promoter of cauliflower mosaic virus and nopaline synthase termination sequence of A. tumefaciens. Incorporation of the aroA target gene confers resistance to the herbicide glyphosate. To increase transgenic line resistance to glyphosate single nucleotide mutation was introduced into the aroA sequence by the site-directed mutagenesis.

LMO characteristics

Modified traits

Resistance to antibiotics
- Kanamycin
Resistance to herbicides
- Glyphosate