Record details

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Living Modified Organism identity

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LMO name

Grape vine modified for the expression of a marker gene

Transformation event

6 independent lines

Developer(s)

Record #101384

BAZ

Institut für landwirtschaftliche Kulturen

Bundesanstalt für Züchtungsforschung an Kulturpflanzen (BAZ)

Erwin-Baur-Str. 27

Quedlinburg

Germany, 06484

Phone:	+49 (0)3946 47 701
Fax:	+49 (0)3946 47 255
Email:	zl@jki.bund.de
Url:	Julius-Kühn-Institut

Description

Vitis vinifera was modified to constitutively express the Escherichia coli beta-glucuronidase. The modification is used to assess the rate of grapevine out-crossing through by the histochemical detection of the GUS activity.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #101177

Vitis vinifera - Grape Vine

Characteristics of the transformation process

Vector

p35Sgus-int

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

CaMV 35S promoter

#100287

0.00 Kb

Beta-Glucuronidase coding sequence

#46004

0.00 Kb

CaMV 35S terminator

#100290

0.00 Kb

Nopaline Synthase Gene Promoter

#100270

0.00 Kb

Neomycin Phosphotransferase II

#15001

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Further details

LMO characteristics

Modified traits

Resistance to antibiotics
- Kanamycin
- Neomycin
Selectable marker genes and reporter genes

Common use(s)

Research