| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
MON-87427-7×MON-89Ø34-3×SYN-IR162-4×MON-87419-8×MON-ØØ6Ø3-6 - Herbicide tolerant, Insect resistant maize
Record information and status
Record ID
114650
Status
Published
Date of creation
2019-04-25 13:16 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-04-25 13:16 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide tolerant, Insect resistant maize
Transformation event
MON87427×MON89034×MIR162×MON87419×NK603
Unique identifier
MON-87427-7×MON-89Ø34-3×SYN-IR162-4×MON-87419-8×MON-ØØ6Ø3-6
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The maize was modified for herbicide tolerance and insect resistance.

Herbicide tolerance
The insertion of CP4 EPSPS confers tolerance to the herbicide glycophosate. Dicamba monooxygenase (DMO) and Phosphinothricin N-acetyltransferase (PAT) genes confer resistance to the herbicides Dicamba and Glufosinate, respectively.

Insect resistance
This maize expresses CRY1A.105, CRY2AB2, and VIP3AA20 for resistance to certain lepidopteran pests such as armyworm, beet armyworm, fall armyworm (Spodoptera sp.), black cutworm (Agrotis ipsilon), european corn borer (Ostrinia nubilalis), western bean cutworm, and the corn earworm (Helicoverpa zea).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Related LMOs
MON-87427-7 - Maize modified for tissue selective glyphosate tolerance
Resistance to herbicides - Glyphosate
Show detection method(s)
MON-89Ø34-3 - YieldGard™ VT Pro™
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
SYN-IR162-4 - Agrisure™ Viptera maize
Mannose tolerance Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
MON-87419-8 - Dicamba and Glufosinate Tolerant Maize
Resistance to herbicides - Glufosinate
MON-ØØ6Ø3-6 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMAP1043;  PV-ZMIR245; pNOV1300; PV-ZMHT507801; PV-ZMGT32
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ti plasmid left border repeat
0.24 Kb
 
 
CaMV Enhanced 35S promoter
0.30 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.06 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Cry1A.105
3.53 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.21 Kb
 
 
FMV 35S promoter
0.56 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Transit peptide and first intron of Rubisco SSU
0.40 Kb
 
 
Cry2Ab2
1.91 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ti plasmid right border repeat
0.23 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Vegetative insecticidal protein 3Aa20
2.37 Kb
 
 
Phosphoenolpyruvate carboxylase, intron 9
0.11 Kb
 
 
CaMV 35S terminator
0.07 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ubiquitin gene promoter
1.64 Kb
 
 
Ubiquitin leader sequence
0.10 Kb
 
 
Ubiquitin Intron Sequence
1.04 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
RA5B gene terminator
0.25 Kb
 
 
PC1SV Promoter
0.43 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.06 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Chloroplast Transit Peptide 4
0.22 Kb
 
 
Dicamba monooxygenase gene
1.02 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.21 Kb
 
 
Rice actin 1 gene promoter
0.80 Kb
 
 
Rice actin 1, intron
0.60 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
CaMV Enhanced 35S promoter
0.60 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Genetic elements from PV-ZMAP1043
Transcription of 5-enolpyruvylshikimate-3-phosphate synthase (cp4 epsps) from Agrobacterium tumefaciens commences from the Cauliflower Mosaic Virus (CaMV) enhanced 35S promoter and ends at the A. tumefaciens nopaline synthase (nos) gene terminator. The transcript contains a Zea mays heat shock protein 70 (Hsp70) intron, Arabidopsis thaliana N-terminal chloroplast transit peptide sequence, and cp4 epsps.

Note:
- Southern blot analyses indicate that a single copy of the T-DNA was inserted at a single site in the parental maize genome and no plasmid vector backbone sequences were detected to have been integrated. DNA sequencing analyses further indicated that the expected T-DNA sequences were integrated.
-The cp4 epsps coding sequence is the codon optimized coding sequence of the aroA gene from Agrobacterium sp. strain CP4 encoding CP4 EPSPS.

Genetic elements from PV-ZMIR245
Two insecticidal protein expression cassettes were inserted into the genome. Bacillus thuringiensis Cry1A.105 expression is under the control of the CaMV 35S enhanced promoter, which first transcribes wheat (Triticum aestivum) 5' untranslated region of the chlorophyll a/b-binding protein (cab) and a rice actin 1 intron before transcribing cry1A.105. Transcription terminates at the wheat heat shock protein 17.3 terminator. Expression of the B. thuringiensis Cry2Ab2 gene starts at the Figwort Mosaic Virus (FMV) promoter, which transcribes the Zea mays heat shock protein 70 (Hsp70), then the Z. mays transit peptide and the Cry2Ab2 coding sequence, before terminating at the nos terminator.

Note:
- The Cry2Ab2 coding sequence was modified for optimal expression in plants.
- South blot analysis confirmed that single insertions of both cry2Ab2 and cry1A.105, as well as no vector backbone were present and in the parent.
- A deletion removed the duplicated enhancer elements compared to the original CaMV e35S promoter in PV-ZMIR245.
- The selectable marker, nphII, cassette was bred out of the parental line and became not associated with this transformation event.


Genetic elements from pNOV1300
In the parental MIR162 maize, a variant of the native B. thuringiensis vegetative insecticidal protein 3Aa (Vip3Aa), named vip3Aa19, which has codon changes that result in a single  M129I amino acid substitution was inserted into the transformation cassette. During the transformation process an additional DNA mutation resulted in a K284Q amino acid substitution. This final form was designated the name Vip3Aa20. Transcription of Vip3Aa20 commences at the Z. mays ubiquitin gene promoter and then transcribes Vip3Aa20 followed by intron 9 of Z. mays phosphoenolpyruvate carboxylase, before terminating at the CaMV 35S terminator.
A second expression cassette, containing the E. coli phosphomannose isomerase gene, was also inserted into the parental genome. The gene is under the control of another ubiquitin promoter and transcription terminates at the Agrobacterium tumefaciens nopaline synthase gene terminator.

Note:
- Southern blot analyses demonstrated that the T-DNA insert contains: i) single copies of a vip3Aa20 gene and a pmi gene; ii) two copies of the ZmUbiInt promoter; iii) one copy of the NOS terminator; and iv) no backbone sequences from transformation plasmid pNOV1300.

Genetic elements from PV-ZMHT507801
The Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (PAT) gene is under the control of the Andropogon gerardii ubiquitin (Ubq) gene promoter and the O. sativa alpha-amylase/trypsin inhibitor terminator. The transcript includes the A. gerardii 5' untranslated leader sequence and an intron from Ubq before the PAT.

The Stenotrophomonas maltophilia Dicamba monooxygenase (DMO) gene is under control of the peanut chlorotic streak caulimovirus (PC1SV) Full-length transcipt (FLt) promoter and the Triticum aestivum (wheat) heat shock protein 17 terminator. The transcript produced contains the wheat chlorophyll a/b-binding 5' untranslated leader sequence (for improved gene expression), the O. sativa Actin 1 untranslated region (UTR) and intron (for improved gene expression), the targeting and UTR of Petunia hybrida Chloroplast Transit Peptide 4, and DMO.

Note:
- Originally, the plasmid vector contained two T-DNA elements that were inserted during the initial transformation event: one containing the dmo and pat expression cassettes, and a second containing a cp4 epsps expression cassette. The cp4 epsps expression cassette is regulated by the Ract1 promoter from Oryza sativa, the Ract1 5′ untranslated leader from Oryza sativa, the Ract1 intron from Oryza sativa, the CTP2 targeting sequence from Arabidopsis thaliana, and the nos 3′ untranslated region from Agrobacterium tumefaciens. Subsequent traditional breeding, segregation, selection, and screening were used to isolate those plants that contain the dmo and pat expression cassettes (T-DNA I) and do not contain the cp4 epsps expression cassette (T-DNA II).
- Molecular characterization of MON 87419 indicated that a single copy of T-DNA I was integrated into the maize genome at a single intact locus that includes all expected elements within the insert, with the exception of incomplete Right and Left Border sequences. These analyses also showed no PV-ZMHT507801 backbone elements or T-DNA II sequences were present in the event.

Genetic elements from PV-ZMGT32
The plant expression plasmid vector, PV-ZMGT32 contains two adjacent plant gene expression cassettes each containing a single copy of the cp4 epsps. In the first (5' end) expression cassette, the cp4 epsps gene is under the regulation of the rice actin promoter (P-Ract1) and the rice actin intron (I-Ract1). The second cassette, which is fused to the 3' end of the first, consists of the cp4epsps gene regulated by the CaMV enhanced 35S promoter (e35S) and an intron from the corn heat shock protein 70 (HSP70). Both expression cassettes incorporate the 3'untranslated region of nos for signal polyadenylation.

Note:
- The vector also contains the nptII gene encoding kanamycin resistance allowing selection of bacteria containing the plasmid, and an origin of replication (ori) necessary for replicating the plasmid in Escherichia coli. For the transformation through particle bombardment, a fragment of the vector (obtained after digestion with the restriction enzyme MluI) was used which contains only the two cp4 epsps gene expression cassettes. Therefore, the nptII gene and the origin of replication were not inserted into the parental NK603.
- The parental NK603 contained one insertion site containing a single copy of the linear DNA of PV-ZMGT32 used for transformation. Both cp4 epsps gene cassettes within the single insert which are intact.
LMO characteristics
Modified traits
  • Tolerance to 3,6-dichloro-2-methoxybenzoic acid (Dicamba)
Common use(s)
  • Food
  • Feed
  • Biofuel
Additional Information
Additional Information
Please refer to the parental organism records for additional information.

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Organization1 record
Risk Assessment1 record