Record details

Living Modified Organism identity

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LMO name

Potato Virus Y-resistant Potato

Transformation event

SY233

Unique identifier

TIC-AR233-5

Developer(s)

Record #115052

Tecnoplant SA

Tecnoplant SA
Constitución 4234
(1254) Capital Federal

Buenos Aires, Capital Federal

Argentina

Phone:	+54 11 4909 8138
Fax:	+54 11 4909 8055
Url:	Tecnoplant (en Español)

Description

The potato was modified for resistance to Potato Virus Y infection. The modified potato expresses the viral coat protein to cross-protect the plant from viral inoculation, spread, multiplication and symptom expression. Although the mechanism is not well-defined, likely the production of the coat protein enhances the production of movement proteins and coordinates the formation of viral replication complexes, thus limiting the spread of the viral via cell-to-cell infections. Additionally, the modified potato expresses neomycin phosphotransferase II for kanamycin selection during transformation.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #12106

Solanum tuberosum - Potato, SOLTU

Characteristics of the transformation process

Vector

pBI121

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

Nopaline Synthase Gene Promoter

#100270

0.18 Kb

Neomycin Phosphotransferase II

#15001

0.80 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

CaMV 35S promoter

#100287

0.35 Kb

PVY coat protein

#15020

1.27 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The modified potato (Solanum tuberosum) contains two genetic cassettes for expression of Escherichia coli neomycin phosphotransferase II (nptII) and Potato Virus Y (PVY) coat protein.

Transcription of nptII begins at an Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminates at an A. tumefaciens nos terminator. Neomycin phosphotransferase is a selectible marker used for selecting transformants.

Transcription of PVY coat protein initiates from a Cauliflower Mosaic Virus 35S promoter and terminates at a A. tumefaciens nos terminator. The sequence of the PVY coat protein contains the last 17 codons of the viral replicase gene, the full coat protein sequence and the 3' noncoding region.

Note:
- Southern blot analysis indicated multiple transgene insertions.

LMO characteristics

Modified traits

Resistance to antibiotics
- Kanamycin
Resistance to diseases and pests
- Viruses
  - Potato virus Y (PVY)

Common use(s)

Food

Detection method(s)

Additional information

Western blot analysis indicated that NPTII was present and readily detected from leaf and tuber samples. From leaf extracts, the PVY coat protein was difficult to detect.

PCR analysis confirmed the presence of both nptII and PVY coat protein.

Southern blot analysis indicated three insertions of the PVY coat protein.

Northern blot analysis detected low levels of PVY coat protein transcript in root, stem and leaf tissues. Detection relied on extended autoradiographic exposure.

For more information, kindly refer to the attached article (Bravo-Almonacid et al., 2012)*.

*Note: Methods used in the article are not validated.

Additional Information

Other relevant website address or attached documents

Field testing, gene flow assessment and pre-commercial of transgenic potato for PVY resistance in Argentina.pdf

NCBI: Nucleotide sequence of Potato Virus Y

Euginius: SY233 (TIC-AR233-5)