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Modified Organism
Maize with impaired DNA-repair mechanism
Record information and status
Record ID
115121
Status
Published
Date of creation
2019-08-19 18:03 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-08-19 18:03 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Maize with impaired DNA-repair mechanism
Transformation event
B104 (ATR-A-KO)
Developer(s)
The item to which this reference refers is pending approval.
Description
The maize plants have been edited using the CRISPR/Cas9 system, which introduced a 1272 basepair deletion into the ATR gene, rendering the ATR nonfunctional.

The ATR gene plays a role in the repair of DNA damage. Thus, DNA damage is expected to accumulate to a higher degree due to the faulty repair mechanism. The plants could act as a biosensor for DNA damage caused by different forms of abiotic stress such as drought, heat, or environmental pollution.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Point of collection or acquisition of the recipient organism
Zea Mays inbred line B104
Related LMOs
Maize with impaired DNA-repair mechanism
Impaired DNA repair Sensitivity to DNA damaging agents
Maize with impaired DNA-repair mechanism
Dr. Hilde Nelissen Impaired DNA repair Sensitivity to DNA damaging agents
Maize with impaired DNA-repair mechanism
Impaired DNA repair mechanisms Sensitivity to DNA damaging agents
Characteristics of the transformation process
Vector
pBUN411
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
  • CRISPR-Cas9 technique
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
ATR serine/threonine kinase - Zea mays - Maize, Corn, MAIZE
Impaired DNA repair
Sensitivity to DNA damaging agents
Notes regarding the genetic elements introduced or modified in this LMO
To introduce the CRISPR/Cas9 gene cassette, plants were transformed using Agrobacterium tumefaciens-mediated transformation. The CRISPR/Cas9 machinery caused a 1272 basepair deletion within the coding sequence of the ATR gene, which resulting in a knockout mutation of the ATR gene. Transformants containing the gene cassette and the desired mutation were selected, and by means of conventional crossing with wild-type plants, the CRISPR/Cas9 gene cassette was removed by selecting T1 plants that only contained the desired mutation, but no longer contained the gene cassette (null-segregants). Therefore, the plants in the field trial are not expected to contain foreign genetic material. They should only contain the desired mutation.
LMO characteristics
Modified traits
  • Impaired DNA repair
  • Sensitivity to DNA damaging agents
Other gene(s) whose expression was affected by the transformation
ATR serine/threonine kinase - Zea mays - Maize, Corn, MAIZE
Impaired DNA repair
Sensitivity to DNA damaging agents
How the expression of the gene(s) was affected
The gene has been edited using the CRISPR/Cas9 system resulting in a 1272 basepair deletion. Therefore, the native protein is not expected to be present within the modified plants.
Common use(s)
  • Research
  • Biosensor
Additional Information
Additional Information
Primarily, ATM responds to double stranded breaks, whereas ATR is activated by single stranded DNA and defects in replication fork progression.
Other relevant website address or attached documents

Records referencing this document (3)
IDDescription
3record(s) found
Modified Organism3 records