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Modified Organism
Cassava modified for increased levels of iron and zinc
Record information and status
Record ID
115142
Status
Published
Date of creation
2019-08-23 14:08 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-08-23 14:08 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Cassava modified for increased levels of iron and zinc
Transformation event
D8023-914001
Developer(s)
National Root Crops Research Institute (NRCRI)
Km 8 Ikot Ekpene Road, PMB 7006
Umuahia, Abia
Nigeria
Phone:+234 (0)8168983790,+2349035068714
Email:info@nrcri.gov.ng,Ihuomaumezurumba@yahoo.com
Url:National Root Crops Research Institute
Description
Cassava (Manihot esculenta Crantz) was modified to express Arabidopsis thaliana FERRITIN 1 and IRON-REGULATED TRANSPORTER 1 to increase the levels of iron by 7-18 times and zinc by 3-10 times.

A selectable marker, Escherichia coli neomycin phosphotransferase II, was also included for selection of transformants using kanamycin.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Manihot esculenta - Cassava, Brazilian arrowroot, Yuca, Manioc, Mandioca, MANES
Point of collection or acquisition of the recipient organism
Cassava cultivar: TMS 91/02324
Related LMOs
Cassava modified for increased levels of iron and zinc
Changes in quality and/or metabolite content Increased levels of iron Resistance to antibiotics - Kanamycin Tolerance to abiotic stress Tolerance to excess iron
Viral resistant cassava with increased levels of zinc and iron
Changes in quality and/or metabolite content Increased levels of iron Resistance to antibiotics - Kanamycin Tolerance to abiotic stress Tolerance to excess iron
Viral resistant cassava with increased levels of zinc and iron
National Root Crops Research Institute Changes in quality and/or metabolite content Increased levels of iron Resistance to antibiotics - Kanamycin Tolerance to abiotic stress Tolerance to excess iron
Show detection method(s)
Characteristics of the transformation process
Vector
p8023
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S terminator
0.21 Kb
 
 
Phloem protein 2-A14 3' untranslated region
0.60 Kb
 
 
Iron-regulated transporter 1
1.02 Kb
 
 
Phloem protein 2-A14 promoter
2.76 Kb
 
 
Nopaline Synthase Gene Terminator
0.27 Kb
 
 
Patatin-1 3' untranslated region
0.43 Kb
 
 
Ferritin 1
0.77 Kb
 
 
B33 gene promotor
1.04 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Neomycin Phosphotransferase II
0.80 Kb
 
 
CaMV Enhanced 35S promoter
0.39 Kb
 
 
CaMV Enhanced 35S promoter
0.39 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Iron-regulated transporter cassette:
Transcription of the Arabidopsis thaliana iron-regulated transporter 1 is under control of the A. thaliana phloem protein 2-A14 promoter and 3' untranslated region, as well as the Cauliflower Mosaic Virus 35S terminator.

Ferritin-1 cassette:
Arabidopsis thaliana ferritin-1 is under transcriptional control of the Solanum tuberosum patatin-1 promoter and terminator, as well as the Agrobacterium tumefaciens nopaline synthase (nos) terminator.

Selectable marker cassette:
Transcription of the Escherichia coli neomycin phosphotransferase II occurs from the duplicated enhanced Cauliflower Mosaic Virus 35S promoter and terminates at the nos terminator.

Please note all genetic elements were in the anti-sense orientation.
LMO characteristics
Modified traits
  • Changes in quality and/or metabolite content
  • Selectable marker genes and reporter genes
  • Increased levels of iron
  • Increased levels of zinc
Common use(s)
  • Food
  • Research
Additional Information
Additional Information
Please note the following:
- This line was developed in parallel to another line with the same genetics. The only difference is that the other recipient cultivar was TMS 98/0505.
- The transformation event state is a placeholder. Several lines were developed and are expected to have similar genetics: D8023-985001, D8023-985002 and D8023-985003.
- The transformation event will be updated when more information is available and/or a line has been chosen for commercialization.
Other relevant website address or attached documents

Records referencing this document (3)
IDDescription
3record(s) found
Modified Organism3 records