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Modified Organism
Viral resistant cassava with increased levels of zinc and iron
Record information and status
Record ID
115144
Status
Published
Date of creation
2019-08-23 14:08 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-08-23 14:08 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Viral resistant cassava with increased levels of zinc and iron
Transformation event
D9001-985005
Developer(s)
National Root Crops Research Institute
National Root Crops Research Institute (NRCRI)
Km 8 Ikot Ekpene Road, PMB 7006
Umuahia, Abia State
Nigeria
Phone:+2349035068714
Email:Ihuomaumezurumba@yahoo.com
Description
Cassava (Manihot esculenta Crantz) was modified to express Arabidopsis thaliana FERRITIN 1 and IRON-REGULATED TRANSPORTER 1 to increase the levels of iron and zinc. The modified cassava additionally contains an RNAi cassette to target the coat proteins of Cassava Brown Streak Virus and Ugandan Cassava Brown Streak Virus and convey resistance to these viruses, the causal agents of Cassava brown streak disease.

Previous overepxression of FER1 and IRT1 increased iron and zinc levels by 7-18 times 3-10 times, respectively.

A selectable marker, Escherichia coli neomycin phosphotransferase II, was also included for selection of transformants using kanamycin.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Manihot esculenta - Cassava, Brazilian arrowroot, Yuca, Manioc, Mandioca, MANES
Point of collection or acquisition of the recipient organism
Cassava cultivar: TMS 98/0505
Related LMOs
Viral resistant cassava with increased levels of zinc and iron
Changes in quality and/or metabolite content Increased levels of iron Resistance to antibiotics - Kanamycin Tolerance to abiotic stress Tolerance to excess iron
Cassava modified for increased levels of iron and zinc
Changes in quality and/or metabolite content Increased levels of iron Resistance to antibiotics - Kanamycin Tolerance to abiotic stress Tolerance to excess iron
Cassava modified for increased levels of iron and zinc
Changes in quality and/or metabolite content Increased levels of iron Resistance to antibiotics - Kanamycin Tolerance to abiotic stress Tolerance to excess iron
Cassava brown streak disease-resistant cassava
Resistance to antibiotics - Kanamycin
Show detection method(s)
Characteristics of the transformation process
Vector
p9001
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Nopaline Synthase Gene Terminator
0.27 Kb
 
 
Coat protein
0.90 Kb
 
 
Coat Protein
0.90 Kb
 
 
Pyruvate orthophosphate dikinase, Intron 3
0.78 Kb
 
 
Coat Protein
0.90 Kb
 
 
Coat protein
0.90 Kb
 
 
CsVMV promoter
0.52 Kb
 
 
Phloem protein 2-A14 3' untranslated region
0.60 Kb
 
 
Iron-regulated transporter 1
1.02 Kb
 
 
Phloem protein 2-A14 promoter
2.76 Kb
 
 
Patatin-1 3' untranslated region
0.43 Kb
 
 
Ferritin 1
0.77 Kb
 
 
B33 gene promotor
1.04 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Neomycin Phosphotransferase II
0.80 Kb
 
 
CaMV Enhanced 35S promoter
0.39 Kb
 
 
CaMV Enhanced 35S promoter
0.39 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
RNA interference cassette:
Transcription of the cassette produces a hairpin RNA (hpRNA). RNA polymerase is recruited to the Cassava vein mosaic virus promoter and then transcribes: two segments of Cassava Brown Streak Virus (CBSV) and the Ugandan Cassava Brown Streak Virus (UCBSV) coat protein (sense orientation), the Flaveria trinervia pyruvate orthophosphate dikinase intron 3 (PDK), and CBSV and UCBSV coat protein (anti-sense orientation). Transcription stops at the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The produced RNA then can form a double stranded segment due to the interstrand homology between the CBSV and UCBSV coat protein segments and the flexible linker/loop, PDK. The double stranded segment of the hpRNA is sufficient to trigger an RNA interference (RNAi) response. The host cell machinery then processes the hpRNA into small interfering RNA, which is used to guide the targeted degradation of RNA molecules with sequence homology. In this case, infecting viral transcripts are degraded by the host cell.

Iron-regulated transporter cassette:
Transcription of the Arabidopsis thaliana iron-regulated transporter 1 is under control of the A. thaliana phloem protein 2-A14 promoter and terminator.

Ferritin-1 cassette:
Arabidopsis thaliana ferritin-1 is under transcriptional control of the Solanum tuberosum patatin-1 promoter and terminator.

Selectable marker cassette:
Transcription of the Escherichia coli neomycin phosphotransferase II occurs from the duplicated enhanced Cauliflower Mosaic Virus 35S promoter and terminates at the nos terminator.

Please note all genetic elements were in the anti-sense orientation.
LMO characteristics
Modified traits
  • Changes in quality and/or metabolite content
  • Selectable marker genes and reporter genes
  • Increased iron levels
  • Increased zinc levels
  • Resistance to CBSV
  • Resistance to UCBSV
Common use(s)
  • Food
  • Research
Detection method(s)
Additional information
The hpRNA cassette containing the coat proteins of CBSV and UCBSV is expected to be processed into small interfering RNA between 21-24 nucleotides in length. Due to this processing, proteins are not expected to be produced from this transcript.
Additional Information
Additional Information
Please note:
The transformation event D9001-985005 is a placeholder. A secondary transformation event (D9001-985007) is expected to have similar introduced genetic elements. This field will be updated once more information is available and if one of the events is chosen for commercialization.

Records referencing this document (4)
IDDescription
4record(s) found
Modified Organism4 records