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Information Resource
Record information and status
Record ID
115482
Status
Published
Date of creation
2020-04-07 15:59 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-04-07 16:00 UTC (austein.mcloughlin@cbd.int)

General Information
Title
A convenient renewable surface plasmon resonance chip for relative quantification of genetically modified soybean in food and feed
Author
Alexandra Plácido, Frederico Ferreira-da-Silva, José Roberto S. A. Leite, Noemí de-los-Santos-Álvarez, Cristina Delerue-Matos
Language(s)
  • English
Publication date
2020-02-26
Subject
Summary, abstract or table of contents
The cultivation of genetically modified organisms (GMO) continues to expand worldwide. Still, many consumers express concerns about the use of GMO in food or feed, and many countries have legislated on labelling systems to indicate the presence of GMO in commercial products. To deal with the increased number of GMO events and to address related regulations, alternative detection methods for GMO inspection are required. In this work, a genosensor based on Surface Plasmon Resonance under continuous flow was developed for the detection and quantification of a genetically modified soybean (event GTS 40-3-2). In a single chip, the simultaneous detection of the event-specific and the taxon-specific samples were achieved, whose detection limits were 20 pM and 16 pM, respectively. The reproducibility was 1.4%, which supports the use of the chip as a reliable and cost-effective alternative to other DNA-based techniques. The results indicate that the proposed method is a versatile tool for GMO quantification in food and feed samples.
Thematic areas
Information on Organisms or LMOs
Organism(s) identification
Glycine max - Soybean, Soya bean, Soya, SOYBN
Gene(s) identification
5-enolpyruvylshikimate-3-phosphate synthase gene - Agrobacterium tumefaciens - Agrobacterium
Resistance to herbicides - Glyphosate
LMO(s) identification
MON-Ø4Ø32-6 - Roundup Ready™ soybean
Resistance to herbicides - Glyphosate
Show detection method(s)
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Identifier
https://doi.org/10.1371/journal.pone.0229659
Publisher and its location
PLOS ONE

US Office
PLOS
1160 Battery Street
Koshland Building East, Suite 225
San Francisco, CA 94111
United States
Phone: +1 (415) 624-1200 | Fax: +1 (415) 546-4090

UK/European Office
PLOS
Carlyle House
Carlyle Road
Cambridge, CB4 3DN
United Kingdom
Phone: +44 (0)1223 442 810 | Fax: +44 (0)1223 442 833
Rights
Open access
Format
PDF - 18 pages (2.4 MB)
Source
Peer-reviewed journal
Keywords and any other relevant information
Detection; quantification; soy; Surface Plasmon Resonance; renewable; food; feed