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Record information and status
Record ID
116226
Status
Published
Date of creation
2021-07-22 16:23 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-07-22 16:23 UTC (austein.mcloughlin@cbd.int)

This document is also available in the following languages:
General Information
Title
Detection of genetically modified plants using LAMP (loop-mediated amplification) technologies
Author
Borys V. Sorochynskyi, Б. В. Сорочинський
Author’s contact information
Ukrainian Institute for Plant Variety Examination,
15 Henerala Rodymtseva St., Kyiv, 03041, Ukraine,
e-mail: see attached publication
https://orcid.org/0000-0002-6167-4071
Language(s)
  • English
  • Ukrainian
Publication date
2021-04-06
Subject
Summary, abstract or table of contents
Purpose. Analysis of the current state and experience on the loop-mediated amplification (LAMP) use to detect genetically modified plants. Methods. Literature search and analysis. Results. General information on the current state and use of the genetically modified plants is provided. Despite the wide distribution of genetically modified plants, the attitude towards them in society continues to remain somewhat wary. About 50 countries have introduced mandatory labeling of GM feed and products, provided that their content exceeds a certain threshold. In order to meet labeling requirements, effective and sensitive methods for detecting known genetic modifications in a variety of plant materials, food products and animal feed must be developed and standardized. The most common approaches to the detection of genetically modified organisms (GMOs) are the determination of specific proteins synthesized in transgenic plants and the detection of new introduced genes. Methods for the determination of GMOs based on the analysis of nucleic acids are more common, since such methods have greater sensitivity and specificity than the analysis of protein composition. Polymerase chain reaction (PCR) method is the main method of nucleic acid analysis, which is now wide used for the detection of GMOs. Loop-mediated amplification (LAMP), which can occur at a constant temperature and therefore does not require the use of expensive equipment may be an alternative to the PCR. Scientific articles about the use of the loop-mediated amplification (LAMP) for the detection of genetically modified plants were analyzed. Advantages and disadvantages of the polymerase chain reaction and loop-mediated amplification are compared. Conclusions. The main criteria for applying a method of GMO detection analysis are as follow: its sensitivity, time of reaction, availability and ease to use, cost of reagents and equipment, and the possibility for simultaneous detection of many samples.
Thematic areas
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Identifier
https://doi.org/10.21498/2518-1017.17.1.2021.228209
Publisher and its location
Plant Varieties Studying and Protection
Format
PDF and HTML
Source
Journal publication
Keywords and any other relevant information
targets for detection; PCR; LAMP; detection limit