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Modified Organism
MON-ØØ163-7 - Roundup Ready™ alfalfa
Record information and status
Record ID
15331
Status
Published
Date of creation
2006-07-10 12:40 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2019-08-01 15:38 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-08-01 15:38 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Roundup Ready™ alfalfa
Transformation event
J163
Unique identifier
MON-ØØ163-7
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Medicago sativa, also known as alfalfa or lucerne, is a perennial herbaceous legume. It contains the 5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain CP4 to confer tolerance to glyphosate, the active ingredient in the herbicide Roundup®, and allow producers to use the herbicide a weed control option. Due to the inclusion of the Figwort Mosaic Virus 356S promoter, herbicide tolerance is constitutive and allows for weed control at anytime (early, pre-plant, pre-emergence, and post-emergence).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Medicago sativa - Alfalfa, Lucerne, MEDSV
Point of collection or acquisition of the recipient organism
Proprietary alfalfa clone 'R2336.'
Characteristics of the transformation process
Vector
PV-MSHT4
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S promoter
0.98 Kb
 
 
HSP 70 5' untranslated leader sequence
0.11 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
rbcS-E9 gene terminator
0.63 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Transcription of the herbicide tolerance gene, 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps) from Agrobacterium tumefaciens, is under control of the Figwort Mosaic Virus 35S promoter and the Pisum sativum Rubisco small subunit terminator. The transcript also contains the Petunia hybrid heat shock protein 70 5'untranslated region for enhanced translation and the Arabidopsis thaliana chloroplast transit peptide 2 for targeting the translated polypeptide to the chloroplast. Transcript level are expected to be elevated due to the constitutive, viral promoter and the protein sequestered in the chloroplasts. 

Note:
- A single T-DNA integration site was detected by Southern blot analysis
- The vector backbone was not integrated into the J163 genome
- A single basepair change (A to C) was detected  in the J163 insertion. However, the change occurs in an intervening sequence and thus is not expected to change the sequences transcribed or translated.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
Lucerne is a forage crop for feeding to dairy cattle and horses, as well as a valuable feed crop for beef cattle, sheep and other livestock. It is a nitrogen-fixing crop due to its symbiosis with Rhizobium meliloti and has the ability to improve soil fertility.

The EPSPS gene is present in animals, fungi, bacteria and plants and is responsible for aromatic amino acid biosynthesis. Glycophosate binds to the plant version of the EPSPS gene and blocks enzymatic biosynthesis of the aromatic amino acids and leads to the death of the plant. The introduced gene was isolated from the common soil bacterium Agrobacterium tumefaciens strain CP4 and the form of EPSPS enzyme produced by this gene has a low affinity for glycophosate, thus allowing for the continued biosynthesis of aromatic amino acids.
Other relevant website address or attached documents

Records referencing this document (16)
IDDescription
16record(s) found
Country's Decision or any other Communication8 records
Information Resource1 record
Modified Organism2 records
Risk Assessment5 records