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Modified Organism
BPS-A1Ø2Ø-5 - Amadea potato
Record information and status
Record ID
48075
Status
Published
Date of creation
2009-01-09 13:15 UTC (manoela.miranda@cbd.int)
Date of last update
2020-04-29 15:42 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-04-29 15:42 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Amadea potato
Transformation event
AM04-1020
Unique identifier
BPS-A1Ø2Ø-5
Developer(s)
BASF Plant Science GmbH
Carl-Bosch-Str. 38
Ludwigshafen
Germany, 67056
Phone:+49 621 60-0
Fax:+49 621 60-42525
Email:global.info@basf.com
Url:BASF AG - Pflanzenbiotechnologie
Description
The potato was modified for increased amylopectin and decreased amylose content. To achieve the reduction in amylose, an RNA interference cassette was introduced into the genome. The cassette encodes inverted repeats of a portion of granule bound starch synthase (gbss). The formation of hairpin RNA (hpRNA; double-stranded structure) triggers an RNAi response that silences the expression of the endogenous gbss through targetted degradation of the transcript and thus preventing the production of the GBSS protein. The reduction in enzyme activity resulted in a 98% increase in amylopectin content in the modified potato. Additionally, the insertion contains an Arabidopsis thaliana acetohydroxyacid synthase selectable marker for imidazolinone herbicide selection during transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Potato cultivar Kuras
Characteristics of the transformation process
Vector
Derivate of pPZP200
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Granule bound starch synthase gene promoter
1.00 Kb
 
 
Granule-bound starch synthase gene
0.46 Kb
 
 
Granule bound starch synthase spacer
0.07 Kb
 
 
Granule-bound starch synthase gene
0.46 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Nopaline Synthase Gene Promoter
0.29 Kb
 
 
Acetohydroxy acid synthase gene
2.01 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
RNA interference cassette:
Transcription begins at the Solanum tuberosum granule bound starch synthase (gbss) promoter and terminates at the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The transcript is contains (from 5' to 3'): a sense portion of gbss, gbss spacer region and an anti-sense portion of gbss. After transcription, the sense and anti-sense regions will base-pair to form a hairpin structure, which can elicit an RNA interference response. The spacer acts as a connecting loop to allow for the formation of this secondary structure. The transcript is not expected to be translated due to RNA interference cellular processing. For more information, kindly refer to the "Other gene(s) whose expression was affected by the transformation" section below.

Selectable marker:
Transcription of Arabidopsis thaliana acetohydroxyacid synthase commences from a nos promoter and terminates at a nos terminator.

Notes:
- Southern blot analysis indicated a single insertion of the T-DNA in the potato genome.
- The nos terminator of acetohydroxyacid synthase was truncated during transformation, but this deletion does not seem to affect the expression of the coding sequence. In the vector, the terminator was 253 basepairs.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Other gene(s) whose expression was affected by the transformation
Granule-bound starch synthase gene - Solanum tuberosum - Potato, SOLTU
altered carbohydrate composition: increased amylopectin content
How the expression of the gene(s) was affected
After transcription, the sense and antisense regions of the transcript will base-pair and form hairpin RNA (hpRNA). The double-strandedness is recognized by DICER, which will fragment the hpRNA into small interfering RNA (siRNA) of roughly 21 to 23 basepairs in size. These siRNAs will then complex with ARGONAUTE family proteins, which will unwind the siRNA duplex, leaving a single strand of the siRNA and activating the RISC complex. Using the complexed single strand of the siRNA, the RISC complex will seek out transcripts with complementarity to the siRNA. Transcripts with complementary sequences will be targetted degradation. Thus, the cellular RNAi machinery will target the endogenous granule bound starch synthase, resulting in the silencing of gene expression (reduction of mRNA and protein).
Common use(s)
  • Food
Detection method(s)
External link(s)

Records referencing this document (12)
IDDescription
12record(s) found
Country's Decision or any other Communication3 records
Information Resource1 record
Modified Organism3 records
Organization1 record
Risk Assessment4 records