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Modified Organism
BPS-PHØ48-1 - Fortuna potato
Record information and status
Record ID
48362
Status
Published
Date of creation
2009-03-05 12:50 UTC (manoela.miranda@cbd.int)
Date of last update
2020-04-29 20:36 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-04-29 20:36 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Fortuna potato
Transformation event
PH05-026-0048
Unique identifier
BPS-PHØ48-1
Developer(s)
BASF Plant Science GmbH
Carl-Bosch-Str. 38
Ludwigshafen
Germany, 67056
Phone:+49 621 60-0
Fax:+49 621 60-42525
Email:global.info@basf.com
Url:BASF AG - Pflanzenbiotechnologie
Description
The potato was modified for resistance to Phytophthora infestans through the expression of the resistance (R) genes Rpi-blb1 and Rpi-blb2 from a wild relative Solanum bulbocastaneum. The R genes encode nucleotide-binding site-leucine rich repeat type proteins and play a role in host defence against the pathogenic fungus.

Additionally, a selectable marker, Arabidopsis thaliana acetohydroxyacid synthase, was included for imidazolinone selection during transformation. The enzyme carries a point mutation, S653N, which confers herbicide tolerance.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Characteristics of the transformation process
Vector
Vector VCPMA16 derived from pPZP200
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Phytophthora infestans Resistance gene 2 Promoter
1.53 Kb
 
 
Phytophthora infestans Resistance gene 2
3.89 Kb
 
 
Phytophthora infestans Resistance gene 2 Terminator
2.53 Kb
 
 
Phytophthora infestans Resistance gene 1 Promoter
1.17 Kb
 
 
Phytophthora infestans Resistance gene 1
3.59 Kb
 
 
Phytophthora infestans Resistance gene 1 Terminator
0.41 Kb
 
 
Nopaline Synthase Gene Promoter
0.29 Kb
 
 
Acetohydroxy acid synthase gene
2.01 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Expression cassettes:
The insertion contains three gene expression cassettes: Solanum bulbocastaneum Phytophthora infestans resistance gene 1 (Rpi-blb1), Solanum bulbocastaneum Phytophthora infestans resistance gene 2 (Rpi-blb2) and Arabidopsis thaliana acetohydroxyacid synthase (ahas).

Transcription of Rpi-blb1 and Rpi-blb2 are under control of the native promoters and terminators. Transcription of ahas is under control of the Agrobacterium tumefaciens nopaline synthase promoter and terminator. The R gene cassettes were originally excised from the donor genome as genomic fragments and inserted into the vector.

The coding sequence of ahas contains a point mutation S653N (serine to asparagine at amino acid position 653), which confers herbicide tolerance.
LMO characteristics
Modified traits
  • Phytophthora infestans resistance
Common use(s)
  • Food
Detection method(s)
Additional information
Low level of expression of both Rpi-blb1 and Rpi-blb2 was detected by real-time PCR analysis in leaves, stems, tubers and roots. In flowers, low expression of Rpi-blb2 was detected. However, the expression of Rpi-blb1 was not detected in floral tissues.

Due to the nos promoter, low expression levels in all parts of the plant are expected for ahas. Expression of ahas was essential for the selection of transformants during tissue culturing.
Additional Information
Additional Information
Vector information
Derivate of pPZP200. Reference: Hajdukiewicz et al (1994) Plant Mol. Biol., 25, 989-994.
Other relevant website address or attached documents

Records referencing this document (7)
IDDescription
7record(s) found
Country's Decision or any other Communication3 records
Modified Organism1 record
Risk Assessment3 records