The canola contains one gene cassette for Bacillus
licheniformis glyphosate-N-ccteyltransferase (gat).
The coding sequence was under control of an Arabidopsis
thaliana ubiquitin-10 promoter and a Solanum
tuberonsum proteinase inhibitor II 3' untranslated
The gat coding sequence expresses the GAT4621 version of
the protein, which was functionally improved by a gene shuffling
process to optimize the kinetics of glyphosate
Southern blot analysis indicated that a single, intact PHP28181 DNA
fragment was inserted into the genome with no plasmid backbone DNA.