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Maize modified for herbicide tolerance and insect resistance
MON-87411-9 maize was modified to contain a suppression cassette
that expresses an inverted repeat sequence designed to match the
sequence of western corn rootworm (WCR).
The expression of the suppression cassette results in the formation
of a double-stranded RNA (dsRNA) transcript containing a 240 bp
fragment of the WCR Snf7 gene (DvSnf7). Upon consumption, the
plant-produced dsRNA in MON 87411 is recognized by the CRW's RNA
interference (RNAi) machinery resulting in down regulation of the
targeted DvSnf7 gene leading to CRW mortality.
MON 87411 also contains a cry3Bb1 gene that produces a modified
Bacillus thuringiensis (subsp. kumamotoensis) Cry3Bb1 protein to
protect against CRW larval feeding.
In addition, MON 87411 contains the cp4 epsps gene from
Agrobacterium sp. strain CP4 that encodes for the
5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein, which
confers tolerance to glyphosate
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
- Agrobacterium-mediated DNA transfer
CaMV Enhanced 35S promoter
rbcS-E9 gene terminator
5' untranslated leader from chlorophyll a/b-binding protein
Heat shock protein 17.3 terminator
Alpha Tubulin Gene promoter
Chloroplast transit peptide 2
5-enolpyruvylshikimate-3-phosphate synthase gene
Alpha Tubulin Gene terminator
The DvSnf7p sequence is the partial coding sequence of the Snf7
gene from Diabrotica virgifera virgifera encoding the SNF7 subunit
of the ESCRT-III complex. The DvSnf7 suppression cassette contains
two 240 bp DvSnf7p sequences in an inverted orientation. There is
an intervening sequence of 150 nucleotides between the two DvSnf7p
sequences. When the suppression cassette is transcribed, the RNA
expressed forms a hairpin loop thereby allowing the formation of
double stranded DvSnf7 RNA. The DvSnf7p sequences in the
suppression cassette produce a 240 bp dsRNA that upon transcription
triggers the RNAi mechanism.
Sequencing, PCR, and bioinformatic analysis indicated that a
single, intact insert of the DvSnf7 suppression cassette and the
cry3Bb1 and cp4 epsps expression cassettes were stably integrated
into the maize genome. Analyses also indicated the absence of the
plasmid backbone DNA in MON 87411 maize.
- Resistance to diseases and pests
- Coleoptera (beetles)
- Western corn rootworm (Diabrotica virgifera)
- Northern corn rootworm (Diabrotica barberi)
- Resistance to herbicides