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Maharashtra Hybrid Seeds Company Limited (Mahyco)
Jalna - Aurangabad Road,
Post Box no. 76,
The cauliflower (Brassica oleracea) was modified for
resistance to Lepidoptera pests through the introduction and
expression of Bacillus thuringiensis crystal protein,
Cry1Ac. The protein forms pores in the midgut lining of susceptible
pests, leading to cell lysis and septicemia. Additionally, the
cauliflower contains an Escherichia coli neomycin
phosphotransferase II cassette for kanamycin selection during
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica oleracea var. botrytis - Cauliflower, Broccoflower, Calabrese, Romanesci broccoli
Brassica oleracea Botrytis group
- Agrobacterium-mediated DNA transfer
Neomycin Phosphotransferase II
CaMV Enhanced 35S promoter
The modified cauliflower contains two gene cassettes:
Escherichia coli neomycin phosphotransferase II
(nptII) and Bacillus thuringiensis cry1Ac.
Transcription of nptII commences from a Cauliflower
mosaic virus (CaMV) 35S promoter and terminates at a CaMV 35S
terminator. Due to the viral promoters, transcription of
nptII is expected to occur at elevated levels in all plant
Transcription of cry1Ac is controlled by a CaMV enhanced
35S promoter. Transcription is expected to occur at very high
levels in all tissues due to the enhanced nature (duplicated
enhancer) of the viral promoter.
- Resistance to antibiotics
- Resistance to diseases and pests
- Lepidoptera (butterflies and moths)
See provision patent attached in the 'Additional Information'