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Record information and status
Record ID
Date of creation
2021-03-23 19:29 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-03-23 19:29 UTC (austein.mcloughlin@cbd.int)

General Information
Two FAST multiplex real-time PCR reactions to assess the presence of genetically modified organisms in food
Geoffrey Cottenet, Carine Blancpain, Véronique Sonnard, Poh Fong Chuah
Author’s contact information
Geoffrey Cottenet
Institute of Food Safety & Analytical Science,
Nestec Ltd., Nestlé Research Center,
Vers-chez-les-Blanc, Lausanne, Switzerland

Kindly refer to the article for contact information
  • English
Publication date
Summary, abstract or table of contents
Methods to detect the presence of genetically modified organisms (GMOs) are evolving constantly to comply with legislation and include new transgenic traits developed by biotechnology companies. Since traditional screening methods target only a limited number of markers, not all GM-events can be detected easily. To cover a broader range, a new GMO screening method was developed, based on two real-time PCR reactions, targeting i) six major GM-markers and ii) six GM-events that do not contain these markers. This method used the FAST PCR technology and allowed a further reduction in time-to-result. Using a wide array of reference materials and proficiency test samples, the method showed a broad screening capacity and the absolute limit of detection was consistently below 20 copies, thereby demonstrating the method is fit-for-purpose.
Thematic areas
Information on Organisms or LMOs
Gene(s) identification
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium
FMV 34S promoter - Figwort mosaic virus - Figwort mottle virus, FMV, CMoVb
Phosphinothricin N-acetyltransferase gene - Streptomyces hygroscopicus - STRHY
Resistance to herbicides - Glufosinate
rbcS-E9 gene terminator - Pisum sativum - Garden pea, PEA
Cry1Ab/Ac - Bacillus thuringiensis - Bt, Bacillus, BACTU
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) - Cotton bollworm (Helicoverpa spp.)
LMO(s) identification
DAS-4Ø278-9 - Enlist™ Maize
Dow AgroSciences GmbH Resistance to herbicides Tolerance to 2,4-Dichlorophenoxyacetic acid Tolerance to aryloxyphenoxypropionate
Show detection method(s)
VCO-Ø1981-5 - Glyphosate tolerant maize
Limagrain Resistance to herbicides - Glyphosate
DP-3Ø5423-1 - TREUS™Plenish™ Soybean
Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to herbicides - Sulfonylurea
Show detection method(s)
BPS-CV127-9 - Herbicide-tolerant soybean
BASF S.A. Resistance to herbicides - Imidazolinone, Sulfonylurea
BCS-GHØØ2-5 - GlyTol™ Cotton GHB614
Bayer CropScience Resistance to herbicides - Glyphosate
Show detection method(s)
DP-Ø73496-4 - Optimum® Gly Canola
Resistance to herbicides - Glyphosate
Show detection method(s)
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Publisher and its location
Food Chemistry
PDF - 268 KB (6 pages)
Keywords and any other relevant information
LMO; screening; detection; real-time PCR; multiplex