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800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
|Phone:||+ 1 314 694-1000|
|Fax:||+1 314 694-3080|
Glyphosate herbicide tolerant canola produced by inserting the
epsps gene encoding the enzyme 5-enolypyruvylshikimate-3-phosphate
synthase (EPSPS) and glyphosate oxidase (gox) from Ochrobactrum
anthropi. These modifications confer tolerance to the herbicide
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Cultivar: Westar (Standard variety used in the Western Canadian
Cooperative Rapeseed Test (Co-Op Test))
MON-89249-2 - Roundup Ready™ canola
Resistance to herbicides - Glyphosate
- Agrobacterium-mediated DNA transfer
Chloroplast transit peptide 2
5-enolpyruvylshikimate-3-phosphate synthase gene
rbcS-E9 gene terminator
Glyphosate oxidoreductase gene
rbcS-E9 gene terminator
The Glyphosate oxidoreductase varies from the wild type version of
the gene at 3 amino acid sites (G85S, R153K and R334H) and is
designated as goxv247.
The EPSPS gene was codon optimised for plant expression.
PCR and southern blot analysis indicated that MON-ØØØ73-7 contains
a single insertion event containing one copy of the T-DNA from
plasmid PV-BNGT04. No genetic elements from outside of the right
and left borders of the plasmid were transferred into or are
present in the genomic DNA of the LMO.
The epsps gene codes for the enzyme
5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that is present
in all plants, bacteria and fungi. The epsps gene put into GT73 was
isolated from strain CP4 of the common soil bacterium Agrobacterium
tumefaciens and is a glyphosate tolerant form of EPSPS. The EPSPS
enzyme is part of an important biochemical pathway in plants called
the shikimate pathway, which is involved in the production of
aromatic amino acids and other aromatic compounds. When
conventional canola plants are treated with glyphosate, the plants
cannot produce the aromatic amino acids needed to grow and survive.
EPSPS is not present in mammals, birds or aquatic life forms, which
do not synthesize their own aromatic amino acids. For this reason,
glyphosate has little toxicity to these organisms. The EPSPS enzyme
is naturally present in foods derived from plant and microbial
The canola line GT73 contains a second gene that codes for a
modified version of glyphosate oxidase (GOX) enzyme that is
ubiquitous in nature. The goxv247 gene inserted into GT73 was
isolated from strain LBAA of the bacterium Ochrobactrum anthropi.
Glyphosate oxidase (GOX) enzyme accelerates the normal breakdown of
the herbicide glyphosate into two non-toxic compounds,
aminomethylphosphonic acid (AMPA) and glyoxylate. AMPA is the
principal breakdown product of glyphosate and is degraded by
several microorganisms, while glyoxylate is commonly found in plant
cells and is broken down by the glyoxylic pathway for lipid